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prk5 ha yfp raptor addgene  (Addgene inc)


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    Structured Review

    Addgene inc prk5 ha yfp raptor addgene
    Prk5 Ha Yfp Raptor Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prk5+ha+yfp+raptor+addgene/pSG5-HA-p300+(Plasmid+%2389094)/pm40262613-263-203-204
    Average 93 stars, based on 12 article reviews
    prk5 ha yfp raptor addgene - by Bioz Stars, 2026-10
    93/100 stars

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    shRNA:

    Article Title: Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A
    Article Snippet: MCF10A Horizon #HD PAR-058 MSC ATCC #PCS-500-011 Primary mouse cortical neurons in-house N/A MEF in-house N/A Experimental Models: Organisms/Strains C57BL/6 mice Jackson Laboratories C57BL/6 Oligonucleotides Primer for Raptor K1097R forward primer, 5’-ccaaatcagcaaa attcctccagaccctgatggca-3’ This paper N/A Primer for Raptor K1097R reverse primer, 5’-tgccatcagggtct ggaggaattttgctgatttgg-3’ This paper N/A Primer for Raptor K840R forward primer, 5’-cacggtggccctgt aggcgatgctgttgag-3’ This paper N/A Primer for Raptor K840R reverse primer, 5’-ctcaacagcatcgc ctacagggccaccgtg-3’ This paper N/A For siRNA sequences see Table S1 N/A N/A Recombinant DNA pEFGP Clontech pCMV6-XL5-MCCC1 Origene #SC113201 pcDNA3.1-EP300 Addgene #23252 pcDNA3.1-EP300(HAT-) Addgene #23254 (Continued on next page) Cell Metabolism 29, 192–201.e1–e7, January 8, 2019 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER peYFP-C1-mTOR Addgene #73384 pRK5-HA-YFP-Raptor Addgene #73385 pRK5-HA-YFP-PRAS40 Addgene #73389 pRK5-HA-YFP-DEPTOR Addgene #73390 pRK5-HA-Raptor Addgene #8513 pRK5-HA-GST-RagB Addgene #19301 pLJM1-Flag-RagD Addgene #19316 pLJM1-Flag-Raptor-Rheb15 Addgene #26634 pLKO.1 shRNAs Dharmacon #RHS4080 mouse Mccc1 shRNA Dharmacon #RMM4534-EG72039 Software and Algorithms Prism GraphPad https://www.graphpad.com/ scientific-software/prism/ ImageJ Fiji http://fiji.sc/ IMAGE STUDIO Lite LI-COR Biosciences https://www.licor.com/bio/ products/software/image_studio_lite/ Volocity PerkinElmer http://cellularimaging.perkinelmer.com/ Photoshop Adobe http://www.adobe.com/uk/products/ photoshop.html Other DMEM Sigma Aldrich #D6546 FBS Sigma Aldrich #F7524 FBS, dialyzed Thermo Fisher Scientific #26400-044 L-glutamine Sigma Aldrich #G7513 Penicillin/ streptomycin Sigma Aldrich #P0781 Horse serum Sigma Aldrich #H1270 Hank’s balanced salt solution Sigma Aldrich #H9394 Earle’s balanced salt solution Sigma Aldrich #E7510 leucine Sigma Aldrich #L8000 isoleucine Sigma Aldrich #I2752 valine Sigma Aldrich #V0500 Neurobasal-A medium Thermo Fisher Scientific #12349015 ..

    Software:

    Article Title: Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A
    Article Snippet: MCF10A Horizon #HD PAR-058 MSC ATCC #PCS-500-011 Primary mouse cortical neurons in-house N/A MEF in-house N/A Experimental Models: Organisms/Strains C57BL/6 mice Jackson Laboratories C57BL/6 Oligonucleotides Primer for Raptor K1097R forward primer, 5’-ccaaatcagcaaa attcctccagaccctgatggca-3’ This paper N/A Primer for Raptor K1097R reverse primer, 5’-tgccatcagggtct ggaggaattttgctgatttgg-3’ This paper N/A Primer for Raptor K840R forward primer, 5’-cacggtggccctgt aggcgatgctgttgag-3’ This paper N/A Primer for Raptor K840R reverse primer, 5’-ctcaacagcatcgc ctacagggccaccgtg-3’ This paper N/A For siRNA sequences see Table S1 N/A N/A Recombinant DNA pEFGP Clontech pCMV6-XL5-MCCC1 Origene #SC113201 pcDNA3.1-EP300 Addgene #23252 pcDNA3.1-EP300(HAT-) Addgene #23254 (Continued on next page) Cell Metabolism 29, 192–201.e1–e7, January 8, 2019 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER peYFP-C1-mTOR Addgene #73384 pRK5-HA-YFP-Raptor Addgene #73385 pRK5-HA-YFP-PRAS40 Addgene #73389 pRK5-HA-YFP-DEPTOR Addgene #73390 pRK5-HA-Raptor Addgene #8513 pRK5-HA-GST-RagB Addgene #19301 pLJM1-Flag-RagD Addgene #19316 pLJM1-Flag-Raptor-Rheb15 Addgene #26634 pLKO.1 shRNAs Dharmacon #RHS4080 mouse Mccc1 shRNA Dharmacon #RMM4534-EG72039 Software and Algorithms Prism GraphPad https://www.graphpad.com/ scientific-software/prism/ ImageJ Fiji http://fiji.sc/ IMAGE STUDIO Lite LI-COR Biosciences https://www.licor.com/bio/ products/software/image_studio_lite/ Volocity PerkinElmer http://cellularimaging.perkinelmer.com/ Photoshop Adobe http://www.adobe.com/uk/products/ photoshop.html Other DMEM Sigma Aldrich #D6546 FBS Sigma Aldrich #F7524 FBS, dialyzed Thermo Fisher Scientific #26400-044 L-glutamine Sigma Aldrich #G7513 Penicillin/ streptomycin Sigma Aldrich #P0781 Horse serum Sigma Aldrich #H1270 Hank’s balanced salt solution Sigma Aldrich #H9394 Earle’s balanced salt solution Sigma Aldrich #E7510 leucine Sigma Aldrich #L8000 isoleucine Sigma Aldrich #I2752 valine Sigma Aldrich #V0500 Neurobasal-A medium Thermo Fisher Scientific #12349015 ..

    other:

    Article Title: Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SNCA A53T REV/WT iPS Cell Lines Jackson Laboratories # JIPSC001132 SNCA AST18 iPS Cell Lines Kunath Lab, Chen et al.52 N/A SNCA AST18-2KO iPS Cell Lines Kunath Lab, Chen et al.52 N/A Control midbrain organoids Metzakopian Lab, Patikas et al.53 N/A SNCA A53T midbrain organoids Metzakopian Lab, Patikas et al.53 N/A Experimental Models: Organisms/Strains Zebrafish UAS:Dendra-a-SYN-WT lines (Tg(UAS:Dendra2-Hsa.SNCA, myl7:EGFP)cu49Tg) in-house; Lopez et al.45 N/A Zebrafish UAS:Dendra-a-SYN-A53T lines (Tg(UAS:Dendra2-Hsa.SNCA_A53T, myl7:EGFP)cu50Tg) in-house; Lopez et al.45 N/A Mouse C57 BL/6J Jackson Laboratories #RRID:IMSR_JAX:000664 Mouse mRFP-GFP-LC3 (tfLC3) in-house; Pavel et al.54 N/A PrPsynA53T Tg mice (B6.Cg-Tg(Prnp-SNCA*A53T)23Mkle/J Jackson Laboratories #006823 Oligonucleotides Primer for SNCA Ala53Thr (A53T) mutant; For: 5’-gtcttctcagccactgtcgtcacaccatgcaccactc-3’; Rev: 5’-gagtggtgcatggtgtgacgacagtggctgagaagac-3’ This paper N/A Primer for p300 Ser89Ala (S89A) mutant; For: 5’-catattgaggttaggggaagcaccagatcgcagcaattct-3’ Rev: 5’-agaattgctgcgatctggtgcttcccctaacctcaatatg-3’ This paper N/A Primer for p300 Ser89Asp (S89D) mutant; For: 5’-ccatattgaggttaggggaatcaccagatcgcagcaattctg-3’; Rev: 5’-cagaattgctgcgatctggtgattcccctaacctcaatatgg-3’ This paper N/A Primer for LKB1 Lys48Arg (K48R) mutant; For: 5’-cccccatcaggtacctgccgatgagcttg-3’; Rev: 5’-caagctcatcggcaggtacctgatggggg-3’ This paper N/A ON-TARGETplus Non-targeting siRNA-SMARTpool Dharmacon #D-001810-10 ON-TARGETplus Human EP300 siRNA-SMARTpool Dharmacon #L-003486-00-0005 ON-TARGETplus Human ACLY siRNA-SMARTpool Dharmacon #L-004915-00-0005 ON-TARGETplus Human CBP siRNA-SMARTpool Dharmacon #L-003477-00-0005 ON-TARGETplus Human SNCA siRNA-SMARTpool Dharmacon #L-011109-00-0005 Recombinant DNA pEFGP Clontech N/A pcDNA3.1-myc-6XHis Invitrogen N/A pEGFP-C1-HTT (Q74) Clontech N/A pcDNA3.1-p300 Addgene #23252 pcDNA3.1-p300(HAT-) Addgene #23254 pSG5-HA-p300 Addgene #89094 pRK5-HA-YFP-Raptor Addgene #73385 pRK5-HA-raptor Addgene #8513 pcDNA3-AMPKa2 WT Addgene #15991 pcDNA3-AMPKa2 K45R Addgene #15992 pcDNA3-FLAG-LKB1 Addgene #8590 pEGFP-C1-a-Syn WT Addgene #40822 pEGFP-C1-a-Syn-A53T Addgene #40823 pINDUCER20-a-Syn Addgene #92200 pHM6-httQ74-HA Addgene #40264 pCMV6-Myc-DDK-ACLY Origene #RC200508 (Continued on next page) Neuron 113, 1–17.e1–e13, June 18, 2025 e4



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